Genotyping of rotavirus of neonatal calves by nested-multiplex PCR in India
نویسندگان
چکیده
SARAVANAN, M., M. PARTHIBAN, P. RAMADASS: Genotyping of rotavirus of neonatal calves by nested-multiplex PCR in India. Vet. arhiv 76, 497-505, 2006. ABSTRACT A total of 209 diarrhoeic faecal samples were collected from calves in nine diary cattle herds located in different areas in Tamil Nadu state, India. Nine faecal samples positive for group A rotavirus by polyacrylamide gel electrophoresis (RNA-PAGE) followed by silver staining were analyzed by nested-multiplex-reverse transcription polymerase chain reaction (nested-multiplex PCR) to identify the P and G genotypes. Nested-multiplex PCR was carried out using the type-specific primers of common genotypes P[11],G10 and P[5],G6 to identify their prevalence. Three out of nine samples were found to be P[1],G10 genotype, and none of the samples was P[5],G6 genotype. The occurrence of zoonotic important bovine genotype (P[11],G10) was reported in this study.
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